Mastitis Diagnostic & Bulk Milk Quality

Classic cultural techniques employed by the National Mastitis Council are performed to enumerate and characterize mastitis causing organisms and assess bulk milk quality.

Test Parameters:

  • Ruminant Quarter/Half Culture
  • Bulk tank culture
  • Somatic cell (leukocyte) count

Mastitis Culture

Ruminant Quarter/Half Sampling & Submission:

Note: Composite samples are not recommended.                                                        

                                                                         

  • Using permanent marker, label sterile tubes/vials (Animal ID and quarter - e.g. Bessie RF)  
  • Prelabel tubes for all animals to be sampled
  • Do thorough udder preparation
  • Dry Udder with paper towels or individual clean cloths
  • Wash hands
  • Scrub each teat end with a cotton ball soaked in rubbing alcohol (for each teat use a fresh cotton ball)
  • Dry teat end with dry cotton ball (for each teat use a fresh cotton ball)
  • Discard first squirt of milk
  • Deposit 2nd squirt of milk in sample tube (do not touch inside of cap or sample tube)
  • Tightly recap tube before sampling next quarter, place tube in a clean place
  • Place all tubes/vials in a rack or styrofoam floater for transport, keep cold
  • Freeze samples, if it is not possible to deliver them to the lab the day of sampling
  • Samples should be accompanied by completed submission forms (below), please include your email address and phone number
  • Results will be distributed via email                               

SUBMISSION FORM - Mastitis Quarter Culture

Bulk Tank Culture

Bulk Tank Sampling & Submission:

  • Using permanent marker, label sterile vials
  • Aseptically sample bulk tank, if possible have a licensed sampler take the sample
  • Place vials in styrofoam floater for transport, keep cold
  • Freeze samples, if it is not possible to deliver them to the lab the day of sampling
  • Samples should be accompanied by completed submission forms (below), please include your email address and phone number
  • Results will be distributed via email

SUBMISSION FORM - Bulk Tank Culture

Contact Information

Agency of Agriculture, Food & Markets

116 State Street
Montpelier, Vt 05620-2901
802.828.2430

 

Program Contacts

  • Media Inquiries                      802.622.4662
  • Licensing & Registration        802.828.2436
  • Business Development          802.828.1619
  • Feed, Seed, Fertilizer & Lime  802.828.2431
  • Working Lands                    802.622.4477
  • Public Health & Resource Management                802.828.2431
  • Laboratory                                  802.585.6073
  • Animal Health                        802.828.2421
  • Dairy                              802.828.2433
  • Consumer Protection / Weights & Measures                           802.828.2433
  • Produce Program                  802.522.3132
  • Meat Inspection                    802.828.2426
  • Water Quality                               802.828.2431
    • Farmer Assistance                    802.622.4098
    • Enforcement                                802.828.2431
    • Reports                                      802.272.0323
    • BMP's                                      802.828.3474
  • Act 250                          802.828.2431 
  • Pesticide                        802.828.2431